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p akt 200 monoclonal cell signaling technology  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p akt 200 monoclonal cell signaling technology
    P Akt 200 Monoclonal Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+akt+thr308/Phospho-Akt+(Thr308)+XP+Rabbit+mAb/us12578341-108-22-25
    Average 96 stars, based on 1714 article reviews
    p akt 200 monoclonal cell signaling technology - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Co-Exposure to Bisphenol A and a High-Fat Diet Induces Insulin Resistance via Suppression of Insulin Signaling Molecule Expression and GLUT4 Translocation
    Article Snippet: .. Proteins were incubated with monoclonal antibodies to AKT (CST, Beverly, MA, USA, 4685, 1:1000), p-AKT Ser473 (CST, Beverly, MA, USA, 4060, 1:1000), p-AKT Thr308 (CST, Beverly, MA, USA, 13038, 1:1000), Rab8A (Abcam, Cambridge, UK, ab188674, 1:1000), Rab13 (Merck, Darmstadt, Germany, SAB4200057, 1:1000), Syntaxin4 (Abcam, Cambridge, UK, ab184545, 1:1000), VAMP2 (Abcam, Cambridge, UK, ab181869, 1:5000), GLUT4 (Abcam, Cambridge, UK, ab33780, 1:1000), GSK3β (CST, Beverly, MA, USA, 12456, 1:1000), pGSK3β Ser9 (CST, Beverly, MA, USA, 9322, 1:1000), and GAPDH (Abcam, Cambridge, UK, ab8245, 1:1000),and subsequently incubated with horseradish peroxidase-labeled goat anti-rabbit secondary antibodies. .. Bands were developed, and band density was analyzed with ImageJ 1.46r(USA National Institutes of Health).

    Article Title: Ginsenoside Rh2 Suppresses the Fanconi Anemia Pathway by Inhibiting NF-κB-Mediated FANCL Transcription in Bladder Cancer
    Article Snippet: .. Membranes were incubated with the following primary antibodies at 1:2000 dilution overnight at 4°C: FANCI (Thermo Fisher Scientific, Cat. A300-212 A), FANCD2 (Thermo Fisher Scientific, Cat. MA1-16570), FANCP/SLX4 (Thermo Fisher Scientific, Cat. PA5-45039), FANCQ/XPF (Thermo Fisher Scientific, Cat. PA5-117118), PCNA (Thermo Fisher Scientific, Cat. 13-3900), FANCA (Thermo Fisher Scientific, Cat. 14657), FANCC (Thermo Fisher Scientific, Cat. H00002176-B01P), FANCM (Thermo Fisher Scientific, Cat. PA5-68191), FANCF (Abcam, USA, Cat. ab105266), FAAP24 (Abcam, Cat. ab122800), FAAP100 (Abcam, Cat. ab224178), FANCL (Abcam, Cat. ab272618), FANCB (Abcam, Cat. ab84536), USP1 (Cell Signaling Technology, USA, Cat. 4933), UAF1/WDR48 (Abcam, Cat. ab97343), GAPDH (Cell Signaling Technology, Cat. 2118), p65 (Cell Signaling Technology, Cat. 8242), p-p65 Ser536 (Cell Signaling Technology, Cat. 3033), β-catenin (Cell Signaling Technology, Cat. 8480), p-β-catenin Ser37 (Cell Signaling Technology, Cat. 9561), Akt (Cell Signaling Technology, Cat. 9272), p-Akt Thr308 (Cell Signaling Technology, Cat. 9275), JNK (Cell Signaling Technology, Cat. 9252), p-JNK Thr183/Tyr185 (Cell Signaling Technology, Cat. 4671), STAT3 (Cell Signaling Technology, Cat. 4904), and p-STAT3 Tyr705 (Cell Signaling Technology, Cat. 9145). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (anti-rabbit IgG or anti-mouse IgG, depending on host species; Cell Signaling Technology, Cat. 7074 for rabbit, Cat. 7076 for mouse) at 1:5000 dilution for 1 h at room temperature.

    Bioprocessing:

    Article Title: Co-Exposure to Bisphenol A and a High-Fat Diet Induces Insulin Resistance via Suppression of Insulin Signaling Molecule Expression and GLUT4 Translocation
    Article Snippet: .. Proteins were incubated with monoclonal antibodies to AKT (CST, Beverly, MA, USA, 4685, 1:1000), p-AKT Ser473 (CST, Beverly, MA, USA, 4060, 1:1000), p-AKT Thr308 (CST, Beverly, MA, USA, 13038, 1:1000), Rab8A (Abcam, Cambridge, UK, ab188674, 1:1000), Rab13 (Merck, Darmstadt, Germany, SAB4200057, 1:1000), Syntaxin4 (Abcam, Cambridge, UK, ab184545, 1:1000), VAMP2 (Abcam, Cambridge, UK, ab181869, 1:5000), GLUT4 (Abcam, Cambridge, UK, ab33780, 1:1000), GSK3β (CST, Beverly, MA, USA, 12456, 1:1000), pGSK3β Ser9 (CST, Beverly, MA, USA, 9322, 1:1000), and GAPDH (Abcam, Cambridge, UK, ab8245, 1:1000),and subsequently incubated with horseradish peroxidase-labeled goat anti-rabbit secondary antibodies. .. Bands were developed, and band density was analyzed with ImageJ 1.46r(USA National Institutes of Health).

    Western Blot:

    Article Title: ADSL deficiency is a secondary mitochondrial disease affecting organelle homeostasis and ERK2/AKT signaling in a linear genotype-phenotype relation
    Article Snippet: .. The primary antibodies used in this study were as follows: p -AKT Thr308 (Cell Signaling Technology #9275, WB 1:1000); AKT (Cell Signaling Technology #9272, WB 1:1000); p -ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology #9101, WB 1:1000); ERK1/2 (Cell Signaling Technology #9102, WB 1:1000); ADSL (C-11) (Santa Cruz Biotechnology #sc-365623, WB 1:1000), GAPDH (6C5) (Santa Cruz Biotechnology #sc-32233, WB 1:1000), HSP90α/β (F-8) (Santa Cruz Biotechnology #sc-13119, WB 1:3000), TOM20 (F-10) (Santa Cruz Biotechnology #sc-17764, WB 1:1000, IF 1:200); UBIQUITIN (Santa Cruz Biotechnology #sc-8017, WB 1:1000); ACTIN (Sigma-Aldrich #A2066, WB 1:3000); OxPhos Human antibody cocktail (Thermo Fisher Scientific #45–8099); SYN (3C11 (anti SYNORF1) Developmental Studies Hybridoma Bank, US, WB 1:1000); Giotto ( was a gift from GL Cestra; https://www.antibodyregistry.org/AB_2892585 , WB 1:3000); HRP (Peroxidase AffiniPure Rabbit Anti-Human IgG (H + L), Jackson ImmunoResearch #309-035-003); Discs large (4F3 anti-discs large, Developmental Studies Hybridoma Bank, US, IF 1:200); LC3B (D11, XP, Cell Signaling Technology #3868, WB 1:1000); Cyclin B1 (Cell Signaling Technology #4138, WB 1:1000); OPA1 (Cell Signaling Technology #80471, WB 1:1000); p -DRP1 Ser616 (Cell Signaling Technology #3455, WB 1:1000); DRP1 (Cell Signaling Technology #8570, WB 1:1000); MFN2 (Santa Cruz Biotechnology #sc-515647, WB 1:1000); FLAG (M2, Sigma-Aldrich #F1804, WB 1:1000). ..

    Ubiquitin Proteomics:

    Article Title: ADSL deficiency is a secondary mitochondrial disease affecting organelle homeostasis and ERK2/AKT signaling in a linear genotype-phenotype relation
    Article Snippet: .. The primary antibodies used in this study were as follows: p -AKT Thr308 (Cell Signaling Technology #9275, WB 1:1000); AKT (Cell Signaling Technology #9272, WB 1:1000); p -ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology #9101, WB 1:1000); ERK1/2 (Cell Signaling Technology #9102, WB 1:1000); ADSL (C-11) (Santa Cruz Biotechnology #sc-365623, WB 1:1000), GAPDH (6C5) (Santa Cruz Biotechnology #sc-32233, WB 1:1000), HSP90α/β (F-8) (Santa Cruz Biotechnology #sc-13119, WB 1:3000), TOM20 (F-10) (Santa Cruz Biotechnology #sc-17764, WB 1:1000, IF 1:200); UBIQUITIN (Santa Cruz Biotechnology #sc-8017, WB 1:1000); ACTIN (Sigma-Aldrich #A2066, WB 1:3000); OxPhos Human antibody cocktail (Thermo Fisher Scientific #45–8099); SYN (3C11 (anti SYNORF1) Developmental Studies Hybridoma Bank, US, WB 1:1000); Giotto ( was a gift from GL Cestra; https://www.antibodyregistry.org/AB_2892585 , WB 1:3000); HRP (Peroxidase AffiniPure Rabbit Anti-Human IgG (H + L), Jackson ImmunoResearch #309-035-003); Discs large (4F3 anti-discs large, Developmental Studies Hybridoma Bank, US, IF 1:200); LC3B (D11, XP, Cell Signaling Technology #3868, WB 1:1000); Cyclin B1 (Cell Signaling Technology #4138, WB 1:1000); OPA1 (Cell Signaling Technology #80471, WB 1:1000); p -DRP1 Ser616 (Cell Signaling Technology #3455, WB 1:1000); DRP1 (Cell Signaling Technology #8570, WB 1:1000); MFN2 (Santa Cruz Biotechnology #sc-515647, WB 1:1000); FLAG (M2, Sigma-Aldrich #F1804, WB 1:1000). ..

    other:

    Article Title: Enhancer regulator MLL4 controls skeletal muscle metabolic efficiency by limiting AMPK-mediated fuel catabolism
    Article Snippet: Two-month-old and 4-month-old male WT and Mll4SET MKO mice ( n = 6–10 mice per group) under CD and HFD condition body composition parameters including fat mass and lean tissue mass were determined via dual-energy X-ray absorptiometry (DEXA) using a Lunar PIXImus II densitometer (GE Healthcare) or nuclear magnetic resonance (NMR) with a MiniSpec LF50 (Bruker Optics), according to the manufacturer’s instructions .

    Article Title: Dietary energy levels regulate feed intake of broilers through the brain-gut axis
    Article Snippet: p-Akt (Thr308) , Cell signaling technology , Cat #9275.



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    Effects of dietary energy levels on the hypothalamic Rac1/PI3K/SF1 pathway. (A) Relative mRNA expression of hypothalamic Total-Rac1, SF1, PI3K, Akt, mTOR; (B) western blotting of different energy levels on the Rac1/PI3K/SF1 signaling pathway in the hypothalamus; (C–H) protein levels of Rac1-GTP, p-PI3K, p-Akt473, p-Akt308, SF1, and p-mTOR; (I) coronal section of the ventromedial hypothalamus (VMH) from LED, MED and HED chickens at day 21 were stained for SF1 with anti-SF1 antibody (green) and for all neuronal nuclei (DAPI, blue). Scale bar, 100 μm; (J) Percentages of VMH region in the coronal sections labeling with the positive SF1 cells (SF1 + cells) and all neuronal nuclei (blue). n = 8 slices from 8 chickens for each group. Rac1, Ras-related C 3 botulinum toxin substrate 1; SF1, steroidogenic factor 1; PI3K, phosphatidylinositol-3-kinase; p-PI3K, phosphorylation phosphatidylinositol-3-kinase; Akt, protein kinase B; p-Akt <t>(Thr308),</t> phosphorylation protein kinase B (Threonine308); p-Akt (Thr473), phosphorylation protein kinase B (Threonine473); mTOR, mammalian target of rapamycin; p-mTOR, phosphorylation mammalian target of rapamycin; VMH, ventromedial nucleus; PVN, paraventricular nucleus; LH, lateral hypothalamus; 3V, third ventricle. Error bars indicate SEM, ns indicates no significance; values are means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Cell Signaling Technology Inc p akt 308
    Effects of dietary energy levels on the hypothalamic Rac1/PI3K/SF1 pathway. (A) Relative mRNA expression of hypothalamic Total-Rac1, SF1, PI3K, Akt, mTOR; (B) western blotting of different energy levels on the Rac1/PI3K/SF1 signaling pathway in the hypothalamus; (C–H) protein levels of Rac1-GTP, p-PI3K, p-Akt473, p-Akt308, SF1, and p-mTOR; (I) coronal section of the ventromedial hypothalamus (VMH) from LED, MED and HED chickens at day 21 were stained for SF1 with anti-SF1 antibody (green) and for all neuronal nuclei (DAPI, blue). Scale bar, 100 μm; (J) Percentages of VMH region in the coronal sections labeling with the positive SF1 cells (SF1 + cells) and all neuronal nuclei (blue). n = 8 slices from 8 chickens for each group. Rac1, Ras-related C 3 botulinum toxin substrate 1; SF1, steroidogenic factor 1; PI3K, phosphatidylinositol-3-kinase; p-PI3K, phosphorylation phosphatidylinositol-3-kinase; Akt, protein kinase B; p-Akt <t>(Thr308),</t> phosphorylation protein kinase B (Threonine308); p-Akt (Thr473), phosphorylation protein kinase B (Threonine473); mTOR, mammalian target of rapamycin; p-mTOR, phosphorylation mammalian target of rapamycin; VMH, ventromedial nucleus; PVN, paraventricular nucleus; LH, lateral hypothalamus; 3V, third ventricle. Error bars indicate SEM, ns indicates no significance; values are means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    Effects of dietary energy levels on the hypothalamic Rac1/PI3K/SF1 pathway. (A) Relative mRNA expression of hypothalamic Total-Rac1, SF1, PI3K, Akt, mTOR; (B) western blotting of different energy levels on the Rac1/PI3K/SF1 signaling pathway in the hypothalamus; (C–H) protein levels of Rac1-GTP, p-PI3K, p-Akt473, p-Akt308, SF1, and p-mTOR; (I) coronal section of the ventromedial hypothalamus (VMH) from LED, MED and HED chickens at day 21 were stained for SF1 with anti-SF1 antibody (green) and for all neuronal nuclei (DAPI, blue). Scale bar, 100 μm; (J) Percentages of VMH region in the coronal sections labeling with the positive SF1 cells (SF1 + cells) and all neuronal nuclei (blue). n = 8 slices from 8 chickens for each group. Rac1, Ras-related C 3 botulinum toxin substrate 1; SF1, steroidogenic factor 1; PI3K, phosphatidylinositol-3-kinase; p-PI3K, phosphorylation phosphatidylinositol-3-kinase; Akt, protein kinase B; p-Akt (Thr308), phosphorylation protein kinase B (Threonine308); p-Akt (Thr473), phosphorylation protein kinase B (Threonine473); mTOR, mammalian target of rapamycin; p-mTOR, phosphorylation mammalian target of rapamycin; VMH, ventromedial nucleus; PVN, paraventricular nucleus; LH, lateral hypothalamus; 3V, third ventricle. Error bars indicate SEM, ns indicates no significance; values are means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Frontiers in Veterinary Science

    Article Title: Dietary energy levels regulate feed intake of broilers through the brain-gut axis

    doi: 10.3389/fvets.2026.1694957

    Figure Lengend Snippet: Effects of dietary energy levels on the hypothalamic Rac1/PI3K/SF1 pathway. (A) Relative mRNA expression of hypothalamic Total-Rac1, SF1, PI3K, Akt, mTOR; (B) western blotting of different energy levels on the Rac1/PI3K/SF1 signaling pathway in the hypothalamus; (C–H) protein levels of Rac1-GTP, p-PI3K, p-Akt473, p-Akt308, SF1, and p-mTOR; (I) coronal section of the ventromedial hypothalamus (VMH) from LED, MED and HED chickens at day 21 were stained for SF1 with anti-SF1 antibody (green) and for all neuronal nuclei (DAPI, blue). Scale bar, 100 μm; (J) Percentages of VMH region in the coronal sections labeling with the positive SF1 cells (SF1 + cells) and all neuronal nuclei (blue). n = 8 slices from 8 chickens for each group. Rac1, Ras-related C 3 botulinum toxin substrate 1; SF1, steroidogenic factor 1; PI3K, phosphatidylinositol-3-kinase; p-PI3K, phosphorylation phosphatidylinositol-3-kinase; Akt, protein kinase B; p-Akt (Thr308), phosphorylation protein kinase B (Threonine308); p-Akt (Thr473), phosphorylation protein kinase B (Threonine473); mTOR, mammalian target of rapamycin; p-mTOR, phosphorylation mammalian target of rapamycin; VMH, ventromedial nucleus; PVN, paraventricular nucleus; LH, lateral hypothalamus; 3V, third ventricle. Error bars indicate SEM, ns indicates no significance; values are means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: p-Akt (Thr308) , Cell signaling technology , Cat #9275.

    Techniques: Expressing, Western Blot, Staining, Labeling, Phospho-proteomics